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Bioreactor seaweed cell culture for production of bioactive oxylipins - and eicosanoids, e.g. 13-hydroxyoctadecatetraenoic acid and 13-hydroxyoctadecatrienoic acid production from Laminaria saccharina

Rorrer G L; Modrell J; Zhi C; Yoo H D; Nagle D N; Gerwick W H
Univ.Oregon-State
Department of Chemical Engineering, Oregon State University, Corvallis, OR 97331, USA.
J.Appl.Phycol. 7, 2, 187-98
1995
Language: English

Abstract: Filamentous cell clumps of the brown alga Laminaria saccharina, isolated from female gametophytes, were cultured in an illuminated bubble column photoreactor in GP2 artificial sea- water nutrient culture medium at 13 deg, with aeration at 0.35 l air/min.l culture. Growth kinetics and biomass productivity data were obtained as a function of incident light intensity (2.4-98 umol photon/sq m.sec) and initial cell density (27-149 mg dry cell wt./l). Maximum cell densities exceeded 1,200 mg dry cell wt./l after 20 days of culture, at optimal conditions of 98 umol photon/sq m.sec and 118 mg dry cell wt./l initial cell density. Qualitative analysis of chloroform-methanol extracts of the cell culture biomass by GC-MS confirmed the presence of the hydroxy fatty acids 13-hydroxyoctadecatetraenoic acid and 13-hydroxyoctadecatrienoic acid, the likely products of 15-lipoxygenase (EC-1.13.11.12)-catalyzed oxidation of linoleic acid or linolenic acid. These results should be useful in oxylipin and eicosanoid production, for possible pharmaceutical application. (32 ref)

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